DNA

Part:BBa_K1321004

Designed by: Xenia Spencer-Milnes   Group: iGEM14_Imperial   (2014-10-10)

N-terminal rfc10_C-terminal rfc25 assembly

This part has not been cloned, it contains the sequence which is made at the beginning of an rfc25 format coding region when the prefix is digested with SpeI and ligated to an XbaI site in an rfc10 backbone. This allows entry of rfc25 format parts as C-terminal fusions with N-terminal rfc10 parts as composite parts correctly.

t ACTAGA TGGCCGGC

NB: this part has been deleted as it already exists in the registry as BioBricks BBa_K13191906, BBa_K1159994.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 10
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None